A simple artificial urine for the growth of urinary pathogens
T Brooks, CW Keevil - Letters in applied microbiology, 1997 - academic.oup.com
T Brooks, CW Keevil
Letters in applied microbiology, 1997•academic.oup.comA simple artificial urine medium (AUM) has been developed which provides conditions
similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide
range of urease‐positive and‐negative urinary pathogens. Liquid AUM supported growth at
concentrations of up to 108 cfu ml− 1, as found in normal urine. Reproducible, steady‐state
growth also occurred over many generations in continuous culture. AUM was capable of
forming crystals and encrustations resembling those found in natural urinary tract infections …
similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide
range of urease‐positive and‐negative urinary pathogens. Liquid AUM supported growth at
concentrations of up to 108 cfu ml− 1, as found in normal urine. Reproducible, steady‐state
growth also occurred over many generations in continuous culture. AUM was capable of
forming crystals and encrustations resembling those found in natural urinary tract infections …
Abstract
A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease‐positive and ‐negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 108 cfu ml−1, as found in normal urine. Reproducible, steady‐state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.
Oxford University Press